Reporter

Part:BBa_K2550201:Design

Designed by: Gaurav Byagathvalli   Group: iGEM18_Lambert_GA   (2018-10-15)


LacZ Reporter Gene (Restriction Cloning Compatible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part sequence consists of a mutation to remove the illegal EcoR1 restriction site inhibiting 3A Assembly. This modified version of LacZ is Biobrick compatible and can now be used for restriction cloning.


Source

This part is based on existing LacZ sequences, including the ones in the part registry, with modifications made to the sequence.

References